• Science

Antibodies

A Laboratory Manual
Author: Edward Harlow,David Lane
Publisher: CSHL Press
ISBN: 9780879693145
Category: Science
Page: 726
View: 3187
Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.

    • Science

Antibodies

A Laboratory Manual
Author: Edward A. Greenfield
Publisher: Anchor Books
ISBN: 9781936113804
Category: Science
Page: 847
View: 6458
"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"--

    • Medical

Antibody Engineering


Author: Roland Kontermann,Stefan Dübel
Publisher: Springer Science & Business Media
ISBN: 9783540413547
Category: Medical
Page: 790
View: 4236
Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered.

    • Science

Phage Display


Author: Carlos F. Barbas,Dennis R. Burton,Jamie K. Scott,Gregg J. Silverman
Publisher: CSHL Press
ISBN: 0879697407
Category: Science
Page: 736
View: 5216
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.

    • Science

Phage Display of Peptides and Proteins

A Laboratory Manual
Author: Brian K. Kay,Jill Winter,John McCafferty
Publisher: Elsevier
ISBN: 9780080538709
Category: Science
Page: 344
View: 4653
Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols. Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. Prepared by experts in the field Provides proven laboratory protocols, troubleshooting, and tips Includes maps, sequences, and sample data Contains extensive and up-to-date references

    • Science

Live Cell Imaging

A Laboratory Manual
Author: Robert D. Goldman,David L. Spector
Publisher: CSHL Press
ISBN: 9780879696832
Category: Science
Page: 631
View: 3978
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.

    • Medical

Making and Using Antibodies

A Practical Handbook, Second Edition
Author: Gary C. Howard,Matthew R. Kaser
Publisher: CRC Press
ISBN: 1439869081
Category: Medical
Page: 458
View: 7164
Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science—both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects of how to make and use antibodies for research along with their commercial and industrial applications. Keeping pace with new developments in this area, all chapters in this new edition have been revised, updated, or expanded. Along with discussions of current applications, new material in the book includes chapters on western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present clear descriptions of basic methods for making and using antibodies and supply detailed descriptions of basic laboratory techniques. Each chapter begins with introductory material, allowing for a better understanding of each concept, and practical examples are included to help readers grasp the real-world scenarios in which antibodies play a part. From the eradication of smallpox to combating cancer, antibodies present an attractive solution to a range of biomedical problems. They are relatively easy to make and use, have great flexibility in applications, and are cost effective for most labs. This volume will assist biomedical researchers and students and pave the way for future discovery of new methods for making and using antibodies for a host of applications.

    • Science

Monoclonal Antibodies

A Manual of Techniques
Author: Heddy Zola
Publisher: CRC Press
ISBN: 9780849364761
Category: Science
Page: 232
View: 578
This book describes, in detail, tested techniques for the produc-tion and use of monoclonal antibodies. It covers those aspects of interest to all scientists working with monoclonal antibodies and presents methods in a step-by-step format for easy refer-ence. The text serves as a laboratory manual; and discusses rationale behind each method, and the choices between methods. It also provides a rational basis where several alternative methods are available.

    • Science

Molecular Cloning

A Laboratory Manual
Author: Joseph Sambrook,David William Russell
Publisher: CSHL Press
ISBN: 9780879695774
Category: Science
Page: 2344
View: 9601
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

    • Science

Protein-protein Interactions

A Molecular Cloning Manual
Author: Erica Golemis
Publisher: CSHL Press
ISBN: 0879697237
Category: Science
Page: 938
View: 1295
Reflecting the various advances in the field, this book provides comprehensive coverage of protein-protein interactions. It presents a collection of the technical and theoretical issues involved in the study of protein associations, including biophysical approaches. It also offers a collection of computational methods for analyzing interactions.

    • Science

A Short Course in Bacterial Genetics

A Laboratory Manual and Handbook for Escherichia Coli and Related Bacteria
Author: Jeffrey H. Miller
Publisher: CSHL Press
ISBN: 9780879693497
Category: Science
Page: 456
View: 7932
University of California, Los Angeles. Introduction to bacterial genetics, including laboratory methods, for advanced students and beginning researchers. Handbook with plastic spiral-bound laboratory manual.

    • Business & Economics

PCR primer

a laboratory manual
Author: Carl W. Dieffenbach,Gabriela S. Dveksler
Publisher: Cold Spring Harbor Laboratory Pr
ISBN: N.A
Category: Business & Economics
Page: 520
View: 4690
This second edition of a much-praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.

    • Antibodies, Monoclonal

Monoclonal Antibodies

Principles and Practice : Production and Application of Monoclonal Antibodies in Cell Biology, Biochemistry, and Immunology
Author: James W. Goding
Publisher: N.A
ISBN: N.A
Category: Antibodies, Monoclonal
Page: 315
View: 2246

    • Science

The Condensed Protocols from Molecular Cloning : a Laboratory Manual


Author: Joseph Sambrook,David William Russell
Publisher: CSHL Press
ISBN: 0879697717
Category: Science
Page: 800
View: 4862
The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.

    • Medical

Human Stem Cell Manual

A Laboratory Guide
Author: Jeanne F. Loring,Suzanne Peterson
Publisher: Academic Press
ISBN: 0123854733
Category: Medical
Page: 619
View: 7919
This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses. • Offers a comprehensive introduction to stem cell biology and culture for medical and biology researchers investigating diagnostics and treatments for various diseases • Presents a historical introduction, discussion of alternative methods, and common pitfalls for basic and advanced experimental strategies • Includes new chapters devoted to iPS cells and other alternative sources for generating human stem cells written by the scientists who made these breakthroughs

    • Science

Transfusion Medicine and Hemostasis

Clinical and Laboratory Aspects
Author: Beth H. Shaz,Christopher D. Hillyer,Mikhail Roshal,Charles S. Abrams
Publisher: Newnes
ISBN: 0123977886
Category: Science
Page: 1014
View: 4817
The second edition of Transfusion Medicine and Hemostasis continues to be the only "pocket-size" quick reference for pathology residents and transfusion medicine fellows. It covers all topics in blood banking, transfusion medicine, and clinical and laboratory based coagulation. Short, focused chapters, organized by multiple hierarchical headings, are supplemented with up to 10 suggested reading citations. This single reference covers essentially all the topics required to meet the goals and objectives of a major program in transfusion medicine and clinical coagulation. New chapters in the coagulation testing section reflect the development of new tests available and their incorporation into clinical practice. Coverage includes essential updates on the importance of new cellular therapies, peripheral blood and bone marrow hematopoietic progenitor cells, as well as cord blood banking and regenerative medicine. The authors also examine advances in the understanding of molecular testing and pathogen reduction in two separate quality control chapters (one for blood centers and one for hospitals). Updated content covers new coagulation tests, cellular therapies, and quality control issues Easy to use, with focused, well-defined chapters in a standardized format throughout Offers quick "cross-reference" lists at the end of each chapter Includes lists of common abbreviations and indexes that cross reference diagnostic, clinical and therapeutic commonalities

    • Science

At the Bench

A Laboratory Navigator
Author: Kathy Barker
Publisher: CSHL Press
ISBN: 9780879697082
Category: Science
Page: 465
View: 521
A clue hidden in a toy ship leads Tintin on a dangerous treasure hunt.

    • Science

Drugs

From Discovery to Approval
Author: Rick Ng
Publisher: John Wiley & Sons
ISBN: 1118907191
Category: Science
Page: 552
View: 3424
The third edition of this best-selling book continues to offer a user-friendly, step-by-step introduction to all the key processes involved in bringing a drug to the market, including the performance of pre-clinical studies, the conduct of human clinical trials, regulatory controls, and even the manufacturing processes for pharmaceutical products. Concise and easy to read, Drugs: From Discovery to Approval, Third Edition quickly introduces basic concepts, then moves on to discuss target selection and the drug discovery process for both small and large molecular drugs. The third edition incorporates the latest developments and updates in the pharmaceutical community, provides more comprehensive coverage of topics, and includes more materials and case studies suited to college and university use. Biotechnology is a dynamic field with changes across R&D, clinical trials, manufacturing and regulatory processes, and the third edition of the text provides timely updates for those in this rapidly growing field.

    • Medical

District Laboratory Practice in Tropical Countries


Author: Monica Cheesbrough
Publisher: Cambridge University Press
ISBN: 9780521676311
Category: Medical
Page: 440
View: 3187
A practical and well-illustrated guide to microbiological, haematological, and blood transfusion techniques. The microbiology chapter focuses on common tropical infections. The haematology chapter deals with the investigation of anaemia and haemoglobinopathies. The blood transfusion chapter provides guidelines on the use of blood and blood substitutes, selection of donors and collection.

    • Science

Drosophila Protocols


Author: William Sullivan,M. Ashburner,R. Scott Hawley
Publisher: CSHL Press
ISBN: 9780879695866
Category: Science
Page: 697
View: 888
This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.